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hUC cells expressed pluripotent markers OCT NANOG KLF and
2018-11-08
hUC TAPI-1 expressed pluripotent markers, OCT4, NANOG, KLF4 and SOX2, but expression levels of all, except KLF4 were significantly lower than human ESCs (Fig. S3). Nevertheless, our results were in agreement with a previous report showing expression of pluripotency markers in cells from peri-natal
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br Materials and methods br Acknowledgments We
2018-11-08
Materials and methods Acknowledgments We would like to thank Dr. Saran Karumbayaram for providing guidance in the culture and expansion of the primate fibroblasts. We would also like to thank Steven Dowdy for depositing the T7-VEE-OKS-iG plasmid (Addgene). This project was funded by NIH grant
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The sorted BMSC and HSPC populations
2018-11-08
The sorted BMSC and HSPC populations were observed to exist in distinct states of the cell cycle, equally important for their functions as stem cells. The ginsenoside rg3 gene expression studies further ascertained the cellular physiology of both the sorted cell populations. Our results also coinci
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We also found clear differences in the amount
2018-11-08
We also found clear differences in the amount of stress fibers between the perivascular derivatives. Abundant stress fibers were found in con-vSMCs located throughout the entire cell body. While hiPSC syn-vSMCs had fewer stress fibers, pericyte derivatives demonstrated the lowest levels of stress fi
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Intestinal epithelial SCs IESCs are roughly categorized as e
2018-11-08
Intestinal epithelial SCs (IESCs) are roughly categorized as either quiescent or active IESCs based in part on the expression of specific markers, including LGR5, Olfm4, Ascl2, BMI1, MTERT, and LRIG1 (Barker et al., 2007; Montgomery et al., 2011; Powell et al., 2012; Sangiorgi and Capecchi, 2008). T
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br Results br Discussion To identify
2018-11-08
Results Discussion To identify global trends in the application of hESCs and hiPSCs in research, we established a curated database of published primary research conducted with these PI-103 manufacturer between 2008 and 2013, and performed a thorough analysis of studies involving only hESCs or
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calpain inhibitor 1 However notwithstanding the above we
2018-11-08
However, notwithstanding the above, we found very little electrophysiological and gene expression evidence that stem cells in the midbrain Aq give rise to new cells that migrate ventrolaterally to become VTA and SNc neurons. Although the electrophysiology of eYFP+ Aq cells was glial cell- or stem ce
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Here we report for the first time that the
2018-11-08
Here, we report for the first time that the mouse eye possesses a primitive c-Kit+ cell that is self-renewing, clonogenic and multipotent, the three critical identifiers of tissue specific stem/progenitor butein (Weissman, 2000). In addition, lineage tracing techniques in vivo demonstrate that the m
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The human GMP was found to yield only neutrophils
2018-11-08
The human GMP was found to yield only neutrophils and not other types of GRs (Görgens et al., 2013). Exactly how monocytes/MΦs and DCs develop downstream of the GMP is intensely studied both in human and mouse. In the mouse, a common monocyte/MΦ/DC progenitor (MDP) has been proposed. The MDP is tho
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br Experimental Procedures br Acknowledgments br
2018-11-08
Experimental Procedures Acknowledgments Introduction Hematopoietic stem hcv protease inhibitor (HSCs) are capable of both self-renewal and production of mature blood lineages. Players in this balanced regulation include transcription factors, cell-cycle regulators, signaling molecules, sur
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br Experimental Procedures br Author Contributions br Acknow
2018-11-08
Experimental Procedures Author Contributions Acknowledgments We thank Cory Abate-Shen, Maho Shibata, and Flaminia Talos for insightful comments on the manuscript. This work was supported by NIHR01DK076602. A.M. and R.T. were supported by post-doctoral fellowships from the DOD Prostate Cance
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Results br Discussion Expansion of the
2018-11-08
Results Discussion Expansion of the NP population is thought to have contributed to the dramatic increase in the surface area of the neocortex (or neocorticalization) observed in higher mammals (Kriegstein et al., 2006; Rakic, 2009; Sun and Hevner, 2014). Thus, maintenance of the NP pool size an
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We have previously shown that
2018-11-07
We have previously shown that PRDM14 is transiently expressed in the inner cell mass (ICM) of blastocysts at embryonic day 3.5 (E3.5) and reactivated in specifying PGCs at approximately E6.25 (Yamaji et al., 2008). Knockout (KO) studies have demonstrated that expression of Prdm14 in the ICM is requi
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br Methods Data on the mean number of languages
2018-11-07
Methods Data on the mean number of languages spoken by the average resident of each country under investigation were taken from Parkvall (2009). Parkvall derived his data from (1) answers to a relevant question asked in each country\'s respective census (n=16); (2) semi-official surveys carried o
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Because a number of the causal
2018-11-07
Because a number of the causal determinants of adverse infant outcomes associated with low SEP are potentially avoidable, strategies that promise even modest improvements warrant serious consideration. In a Cochrane Review (2015) examining randomized trials that compared midwifery-led continuity of
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