• By continuously observing hundreds of individual HSCs

  2018-10-24

  

  By continuously observing hundreds of individual HSCs and their progeny during differentiation, we can exclude a change in cell-cycle length in HSPCs upon GADD45G expression, which was unexpected, because studies on solid tissue toll like receptor reported a G2/M cell-cycle arrest mediated by GADD45

• Our study extended the doxycycline mediated cell

  2018-10-24

  

  Our study extended the doxycycline-mediated cell survival effect to human pluripotent ESC/iPSCs. In addition, we show that hESC/iPSC self-renewal is promoted by doxycycline supplementation. Because microglia and inflammatory cells are not present in hESC/iPSC cultures and the doxycycline effects wer

• Altogether this study shows as proof of

  2018-10-24

  

  Altogether, this study shows as proof of principle that conversion of fibroblasts toward a neural cell fate can be achieved solely by small molecule treatment. We identified CB as potent compound to induce neural cell identity and developed a CB-based approach to efficiently generate Schwann scd1 w

• Compared to treatments with other FGF isoforms

  2018-10-24

  

  Compared to treatments with other FGF2 isoforms, stimulation with 22.5kDa FGF2 results in lower stem cell gene expression in hESCs (Fig. 4d), weaker proliferative activity in human dermal fibroblasts (Fig. 5), and lower relative mitogenic activity in BaF3 terbinafine hydrochloride ectopically expre

• Alternative approaches to LV are non viral

  2018-10-24

  

  Alternative approaches to LV are non-viral vectors such as RNA or DNA, which are considered safer due to their integration-free nature (Hardee et al., 2017). However, manufacturing RNA is expensive and difficult because RNA has a propensity to degrade (Yin et al., 2014). On the other hand, DNA plasm

• Raman microspectroscopy is a marker free method

  2018-10-24

  

  Raman microspectroscopy is a marker-free method that can be used to characterize single cells based on a pattern of molecular vibrational modes, which reflects the composition of intracellular proteins, lipids, nucleic acids, and glucagon receptor (Puppels et al., 1990). Notingher et al. (2004) inv

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-24

  

  Experimental Procedures Author Contributions Acknowledgments We wish to thank Nicole Varain and Donna Colon for their excellent technical support assisting with the fetal sheep transplants. This work was supported by NIH, NHLBIR01HL097623, and the Intramural Pilot Program of Wake Forest Sch

• Active cell proliferation is key for transcription factor in

  2018-10-24

  

  Active cell proliferation is key for transcription factor-induced cell-fate change during cellular reprogramming. Because acute leukemias barely or do not proliferate in vitro, we endeavored to reprogram leukemic B cell lines as well as MLL-rearranged primary blasts induced to proliferate through xe

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-24

  

  Experimental Procedures Author Contributions Acknowledgments We appreciate the expert technical assistance of N. Hayashi, K. Iseki, T. Hashiguchi, W. Ohashi, M. Kawahara, S. Okamoto, and C. Yamada (Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe). We appre

• Introduction The mammalian respiratory tract is normally mai

  2018-10-24

  

  Introduction The mammalian respiratory tract is normally maintained by abundant progenitors that differ in molecular phenotype, differentiation potential, and regulation between airway regions along the proximodistal axis (Hogan et al., 2014; Rackley and Stripp, 2012). Basal cells maintain the pseu

• Such spontaneous Ca transients have

  2018-10-24

  

  Such spontaneous Ca2+ transients have been reported in postnatal mouse cochlear supporting cells (Tritsch et al., 2007), and the Ca2+ signals were suggested to be propagated via gap junctions and hemichannels containing CX26 and CX30 (Anselmi et al., 2008; Schutz et al., 2010). It was also suggested

• To learn what specific genes and biological pathways are

  2018-10-24

  

  To learn what specific genes and biological pathways are involved in the response to pro-oxidant exposure, we performed RNA-seq analysis on 72-hpf embryos after 48 hr of pro-oxidant exposure; the heatmap analysis of unsupervised clustering is shown in Figure 3D. We found 316 genes upregulated (incre

• Fulvestrant While all of the original and

  2018-10-24

  

  While all of the original and refined conventional conditions appear to support primary germ cell populations derived from testes of neonatal or prepubertal pups in which at least some SSCs persist for various periods of time, the impacts of the conditions on derivation and long-term maintenance of

• There is a fast growing interest in

  2018-10-24

  

  There is a fast-growing interest in microglia, as they are increasingly implicated in neurodegenerative disease, neurodevelopmental disorders, and in neuropathic pain. Our iPSC microglia transcribe key Amyloid β-Peptide (1-42) involved in AD, PD, and MND. Several other disease-associated genes, incl

• Measurements of light hadrons produced in heavy ion collisio

  2018-10-24

  

  Measurements of light hadrons produced in heavy ion collisions at RHIC energies indeed indicated the suppression of hadron production by over a factor of five in central Au+Au collisions [6]. Meanwhile, no suppression was observed for the same hadrons and high-energy direct photons in case of d+Au c

15582 records 1033/1039 page Previous Next First page 上5页 10311032103310341035 下5页 Last page